Single-Molecule Characterization of the Interactions between Extracellular Chaperones and Toxic alpha-Synuclein Oligomers
Whiten, D.R., Dezerae, D., Horrocks, M.H., Taylor, C.G., De, S., Flagmeier, P., Tosatto, L., Kumita, J.R., Ecroyd, H., Dobson, C.M., Klenerman, D.
Cell Reports, 23, 3492-3500, 2018.

alpha-Synuclein Oligomers Interact with ATP Synthase and Open the Permeability Transition Pore in Parkinson’s Disease
Ludtmann, M. H. R.*, Angelova, P. R.*, Horrocks, M. H.*, Choi, M. L., Rodrigues, M., Baev, A. Y., Berezhnov, A. V., Yao, Z., Little, D., Banushi, B., Al-Menhali, A.S., Ranasinghe, R.T., Whiten, D.R., Yapom, R., Dolt, K.S., Devine, M.J., Gissen, P., Kunath, T., Jaganjac, M., Pavlov, E.V., Klenerman, D., Abramov, A.Y., S. Gandhi, S. *Joint first authors
Nature Communications, 9 (1), 2293, 2018.

A sticky situation: aberrant protein-protein interactions in Parkinson’s disease
Brown, J.W.P., Horrocks, M.H.
Seminars in Cell & Developmental Biology, Just Published, 2018.

Shedding light on aberrant interactions: A review of modern tools for studying protein aggregates
Kundel, F., Tosatto, L.T., Whiten, D.R., Wirthensohn, D.C., Horrocks, M.H.*, Klenerman, D.*
The FEBS Journal, 1742-4658, 2018.

Detecting RNA base methylations in single cells by in situ hybridization
Ranasinghe, R.T., Challand, M.R., Ganzinger, K.A., Lewis, B.W., Softley, C., Schmied, W.H., Horrocks, M.H., Shivji, N., Chin, J.W, Spencer, J., Klenerman, D.
Nature Communications, 9, 2018.

The small heat shock protein Hsp27 binds alpha-synuclein fibrils, preventing elongation and cytotoxicity
Cox, D., Whiten, D.R., Brown, J.W.P., Horrocks, M.H., San Gil, R., Dobson, C.M., Klenerman, D., van Oijen, A.M., Ecroyd, H.
Journal of Biological Chemistry, 293(12) 4486 –4497, 2018.

Hsp70 Inhibits the Nucleation and Elongation of Tau and Sequesters Tau Aggregates with High Affinity
Kundel, F., De, S., Flagmeier, P., Horrocks, M.H., Kjaergaard, M., Shammas, S.L., Jackson, S.E., Dobson, C.M., Klenerman, D.
ACS Chemical Biology, 13 (3), pp 636–646, 2018.


Nanobodies raised against monomeric alpha-synuclein inhibit fibril formation and destabilize toxic oligomeric species.
Iljina, M., Hong, L, Horrocks, M.H., Ludtmann, M.H., Choi, M.L., Hughes, C.D. Ruggeri, F.S. Guilliams, T., Buell, A.K., Lee, J.E., Gandhi, S., Lee, S.F, Bryant, C.E., Vendruscolo, M., Knowles, T.P.J., Dobson, C.M., De Genst, E., Klenerman, D.
BMC Biology, 15, 57, 2017.

Co-aggregation of κ-Casein and β-Lactoglobulin Produces Morphologically Distinct Amyloid Fibrils.
Raynes, J.K., Crepin, P., Horrocks, M.H., Carver, J.A., Day, L.
Small, 13, 14, 2017.

PEGylated liposomes associate with Wnt3A protein and expand putative stem cells in human bone marrow populations.
Janeczek, AA, Scarpa, E., Horrocks M.H, Tare, R.S., Rowland, C.A., Jenner, D., Newman, T.A., Oreffo, R.O.C., Lee, S.F., Evans, N.D.
Nanomedicine, 12, 8, 2017.


Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping.
Bongiovanni, M.*, Godet, J.*,Horrocks, M.H.*, Tosatto, L., Carr, A.R, Wirthensohn, D.C., Ranasinghe, R.T., Lee, J., Ponjavic, A., Fritz, J.V., Dobson, C.M., Klenerman, D., Lee, S.F.
Nature Communications, 7, 2016.

PSD95 nanoclusters are postsynaptic building blocks in hippocampus circuits.
Broadhead M.J., Horrocks M.H., Zhu F., Muresan L., Benavides-Piccione R., DeFelipe J., Fricker D., Kopanitsa M.V., Duncan R.R., Klenerman D., Komiyama N.H., Lee S.F., Grant S.G.N.
Scientific Reports, 6, 2016.

The remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.
Jonsson P., Southcombe J., Mafalda A., Santos A.M., Fernandes R.A., McColl J., Chang V.C., Godkin A., Dunne P.D., Horrocks M.H., Palayret M., Fugger L., Xu X., Davis S.J., Klenerman D.
PNAS, 113, 20, 2016.

Calcium is a key factor in alpha-synuclein induced neurotoxicity.
Angelova, P., Ludtmann, M., Horrocks, M.H., Negoda, A., Cremades, N., Klenerman, D., Dobson, C.M., Wood, N., Pavlov, E., Gandhi, S., Abramov, A.
Journal of Cell Science, 129, 9, 2016.

Single-molecule imaging of individual amyloid protein aggregates in human biofluids.
Horrocks M. H., Lee S.F., Gandhi S., Magdalinou N.K., Chen S.W., Devine M.J., Toasatto, L., Kjaergaard M., Beckwith J.S., Zetterberg H., Iljina M., Cremades N., Dobson C.M., Wood N.W., Klenerman D.
ACS Chemical Neuroscience, 7, 3, 2016.

A kinetic model of the aggregation of alpha-synuclein provides insights into prion-like spreading.
Iljina,M., Garciaa, G.A., Horrocks, M.H., Tosatto, L., Choi, M.L., Ganzinger, K.A., Abramov, A.Y., Gandhi, S., Wood, N.W., Cremades, N.,Dobson, C.M. , Knowles, T.P.J., Klenerman, D.
PNAS, 113, 9, 2016.


Alpha-synuclein oligomers interact with metal ions to induce oxidative stress and neuronal death in Parkinson's disease.
Deas, E., Cremades, N., Angelova, P.R., Ludtmann, M., Yao, Z., Chen, S., Horrocks, M.H., Banushi, B., Little, D., Devine, M., Gissen P., Klenerman, D., Dobson, C.M., Wood, N., Gandhi, S., Abramov. A.Y.
Antioxidants and redox signaling, 24, 7, 2015.

Single-molecule FRET studies on alpha-synuclein oligomerization of Parkinson's disease genetically related mutants.
Horrocks, M.H., Tosatto, L., Dear, A.J., Knowles, T.P.J., Dalla Serra, M., Dobson, C.M., Klenerman, D.
Scientific Reports, 5, 2015.

Aggregated alpha-synuclein and complex I deficiency: exploration of their relationship in differentiated neurons.
Reeve, A.K., Ludtmann, M.H.R, Angelova, P.R., Simcox, E.M., Horrocks, M.H., Klenerman, D., Gandhi, S., Turnbull, D.M., Abramov, A.Y.
Cell Disease and Death, 6, 2015.

Fast flow microfluidics and single-molecule fluorescence for the rapid characterization of alpha-synuclein oligomers.
Horrocks, M.H., Tosatto, L., Dear, A.J., Garcia, G.A., Iljina, M., Cremades, N., Dalla Serra, M., Knowles, T.P.J., Dobson, C.M., Klenerman, D.
Analytical Chemistry, 87, 17, 2015.

Lipid peroxidation is essential for alpha-synuclein-induced cell death.
Angelova, P.R., Horrocks, M.H., Klenerman D, Gandhi S, Abramov, A.Y., Shchepinov, M.S
Journal of Neurochemistry, 133, 4, 2015.

A mechanistic model of tau amyloid aggregation based on direct observation of oligomers.
Shammas, S. L., Garcia, G. A., Kumar S., A., Kjaergaard M., Horrocks, M.H., Shivji N., Mandelkow, E., Knowles T.P.J., Mandelkow E., Klenerman, D..
Nature Communications, 6, 2015.


Bayesian Inference of Accurate Population Sizes and FRET Efficiencies from Single Diffusing Biomolecules.
Murphy, R.M., Danezis, D., Horrocks, M.H., Jackson, S.E., Klenerman, D.
Analytical Chemistry, 86, 17, 2014.

The changing point-spread function: single-molecule-based super-resolution imaging.
Horrocks, M.H., Palayret, M., Klenerman, D., Lee, S.F.
Histochemistry and Cell Biology, 141, 6, 2014.


Single-molecule measurements of transient biomolecular complexes through microfluidic dilution
Horrocks, M.H., Rajah, L., Jonsson, P., Kjaergaard, M., Vendruscolo, M., Knowles, T.P.J., Klenerman, D.
Analytical Chemistry, 85, 14, 2013.


Ubiquitin chain conformation regulates recognition and activity of interacting proteins.
Ye, Y., Blaser, G., Horrocks, M.H., Ruedas-Rama, M.J., Ibrahim, S., Zhukov, A.A., Orte, A., Klenerman, D., Jackson, S.E., Komander, D.
Nature, 492, 7428, 2012.

Single molecule fluorescence under conditions of fast flow.
Horrocks, M.H., Li, H., Shim, J.-U., Ranasinghe, R.T., Clarke, R.W., Huck, W.T.S., Abell, C., Klenerman, D.
Analytical Chemistry, 84, 1, 2012.


  1. (WO2016001644) Diagnosis and Treatment of Neurodegenerative Disorders