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New super-resolution method for imaging in live cells

posted Sep 5, 2020, 6:04 AM by Mathew Horrocks
We have published a new approach to super-resolution imaging in live cells. Entitled "LIVE-PAINT", the method was developed in collaboration with Lynne Regan's lab in the School of Biology. LIVE-PAINT takes advantage of peptide-protein interactions; the protein-of-interest is tagged with a short peptide sequence, which has a binding partner fused to a fluorescence protein. As the fluorescent protein and peptide transiently bind, a fluorescent burst results, and this is accurately pinpointed with nanometre accuracy. Repeated binding events allow a super-resolution image to be built from this. 

Well done to Curran, Zoe, Louise, Owen and Simon!